Overlay histogram showing HepG2 cells stained with CSB-RA592787A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunoprecipitating PAK2 in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA592787A0HU in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA592787A0HU(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)